科学研究費基盤研究(S) 研究成果
論文
1. Amparyup P, Kondo H, Hirono I, Aoki T,
Tassanakajon A. (2008) Molecular cloning, genomic organization and recombinant
expression of a crustin-like antimicrobial peptide from black tiger shrimp Penaeus monodon. Mol Immunol. 2008
45:1085-1093.
2. Supungul P, Tang S, Maneeruttanarungroj C, Rimphanitchayakit V,
Hirono I, Aoki T, Tassanakajon A. (2007) Cloning, expression and antimicrobial
activity of crustinPm1, a major isoform of crustin, from the black tiger shrimp
Penaeus monodon. Dev Comp Immunol.,
32: 61-70.
3. Maningas MBB, Koyama T, Kondo H, Hirono
I, Aoki T. (2008) A peroxiredoxin from Kuruma shrimp, Marsupenaeus japonicus
inhibited by peptidoglycan. Dev Comp Immunol., 32: 198-203.
4. Maningas MBB, Koyama T, Kondo H, Hirono
I, Taki T, Aoki T. (2008) Essential function of transglutaminase and clotting
protein in shrimp immunity. Mol. Immunol. 45:1269-1275.
5. Darawiroj D, Kondo H, Hirono I, Aoki T. (2008) Immune-related gene
expression profiling of yellowtail (Seriola
quinqueradiata) kidney cells stimulated with conA and LPS using microarray
analysis. Fish Shellfish Immunol. 24: 260-266.
6. Lua DT, Hirono I, Kondo H, Aoki T. (2008) Inhibition of red seabream iridovirus (RSIV)
replication by small interfering RNA (siRNA) in a cell culture system.
Anitiviral Res. 77: 142-149.
7. Santos MD, Kondo H, Hirono I, Aoki T. (2008) Teleostean
IL11b exhibits complementing function to IL11a and expansive involvement in
antibacterial and antiviral responses. Mol. Immunol., in press
8. Dumrongphol
Y, Hirota T, Kondo H,
Aoki T, Hirono I. (2008) Identification of novel genes in Japanese flounder (Paralichthys olivaceus) head kidney
up-regulated after vaccination with Streptococcus
iniae formalin-killed cells. Fish Shellfish Immunol. in press
9. Takano T, Kondo H, Hirono I, Endo M, Taki T, Aoki T. (2007)
Molecular cloning and characterization of Toll-like receptor 9 in Japanese
flounder, Paralichthys olivaceus. Mol
Immunol, 44: 1845-1853.
10. Hirono I, Kondo H, Koyama T, Arma NR, Hwang JY, Nozaki R, Midorikawa
N, Aoki T. (2007) Characterization of Japanese flounder (Paralichthys olivaceus) NK-lysin, an antimicrobial peptide. Fish
Shellfish Immunol., 22:567-575.
11. Piyaviriyakul P, Kondo H, Hirono I, Aoki T. (2007) A novel
immune-type receptor of Japanese flounder (Paralichthys
olivaceus) is expressed in both T and B lymphocytes. Fish Shellfish
Immunol., 22:467-476.
12. Hwang JY, Hirono I, Aoki T. (2007) Cloning and expression of a novel
serine protease from Japanese flounder, Paralichthys
olivaceus. Dev Comp Immunol. 31:587-595.
13. Yasuike M, Kondo H, Hirono I, Aoki T. (2007) Difference in Japanese
flounder, Paralichthys olivaceus gene
expression profile following hirame rhabdovirus (HIRRV) G and N protein DNA
vaccination. Fish Shellfish Immunol., 23:531-541.
14. Wongpanya R, Yasuike Y, Aoki T, Hirono I, Tassanakajon A. (2007)
Analysis of gene expression in haemocyte of shrimp Penaeus monodon challenged with white spot syndrom virus by cDNA
microarray. Science Asia, 33: 165-174.
15. Santos MD, Yasuike M, Kondo H, Hirono I, Aoki T. (2007) A novel
type-1 cytokine receptor from fish involved in the Janus kinase/Signal
transducers and activators of transcription (Jak/STAT) signal pathway. Mol.
Immunol., 344: 355-363
16. Khattiya R, Kondo H, Hirono I, Aoki T. (2007) Cloning, expression
and functional analysis of a novel-chemokine gene of Japanese flounder, Paralichthys olivaceus containing two
additional cysteines and an extra fourth exon. Fish Shellfish Immunol., 22：651-662.
17. Kurobe T, Hirono I, Kondo H, Taki T, Aoki T. (2007) Molecular
cloning, characterization, expression and functional analysis of Japanese
flounder Paralichthys olivaceus Fas
ligand. Dev Comp Immunol. 31: 687-695.
18. Matsuyama T, Fujiwara A, Nakayasu C, Kamaishi T, Oseko N, Tsutsumi
N, Hirono I, Aoki T. (2007) Microarray analyses of gene expression in Japanese
flounder Paralichthys olivaceus
leucocytes during monogenean parasite Neoheterobothrium
hirame infection. Dis Aquat Organ., 75:79-83.
19. Leu JH, Chang CC, Wu JL, Hsu CW, Hirono I, Aoki T, Juan HF, Lo CF,
Kou GH, Huang HC. (2007) Comparative analysis of differentially expressed genes
in normal and white spot syndrome virus infected Penaeus monodon. BMC Genomics. 8:120-133.
20. Matsuyama T, Fujiwara A, Nakayasu C, Kamaishi T, Oseko N, Hirono
I, Aoki T. (2007) Gene expression of leucocytes in vaccinated Japanese flounder
(Paralichthys olivaceus) during the
course of experimental infection with Edwardsiella
tarda. Fish Shellfish Immunol, 22: 598-607.
21. Lua DT, Yasuike M, Hirono I, H. Kondo, Aoki T. (2007)
Transcriptional profile of red seabream iridovirus in a fish model as revealed
by viral DNA microarrays. Virus gene, 35: 449-461.
22. Yazawa R, Kondo H, Hirono I, Aoki T. (2007) Cloning and
characterization of the IkappaBalpha gene from Japanese flounder, Paralichthys olivaceus. Fish Shellfish
Immunol. 23:808-814.
23. Kondo H, Tzeh AGY, Hirono I, Aoki T. (2007) Identification of a
novel C-type lectin gene in Japanese flounder, Paralichthys olivaceus. Fish Shellfish Immunol. 23:1089-1094.
24. Kim HJ, Yasuike M, Kondo H, Hirono I, Aoki T. (2007) Molecular
characterization and gene expression of a CXC chemokine gene from Japanese
flounder Paralichthys olivaceus. Fish
Shellfish Immunol. 23: 1275-128.
25. Kurobe T, Hirono I, Kondoa H, Yamasihta M, Aoki T. (2007)
Molecular cloning, expression, and functional analysis of caspase 10 from
Japanese flounder Paralichthys olivaceus.
Fish Shellfish Immunol. 23: 1266-1274.
26. Hwang JY, Santos MD, Kondo H, Hirono I, Aoki T. (2007)
Identification, characterization and expression of a novel cytokine M17
homologue (MSH) in fish. Fish Shellfish Immunol. 23: 1256-1265.
27.
Caipang CM, Hirono I, Aoki T. (2006) Antigenic components, immunogenicity and
retention of formalin-inactivated red seabream iridovirus (RSIV) vaccine in red
seabream, Pagrus major. Fish
Shellfish Immunol., 20: 597-609.
28.
Takano T, Kondo H, Hirono I, Saito-Taki T, Endo M, Aoki T. (2006)
Identification and characterization of a myeloid differentiation factor 88
(MyD88) cDNA and gene in Japanese flounder, Paralichthys olivaceus. Dev Comp
Immunol. 30: 807-816.
29.
Ooi EL, Hirono I, Aoki T. (2006) Functional characterization of the Japanese
flounder Paralichthys olivaceus, Mx
promoter. Fish Shellfish Immunol., 21: 293-304.
30.
Yazawa, R., Hirono, I, Aoki, T. (2006) Transgenic zebrafish expressing chicken
lysozyme show resistance against bacterial diseases. Transgenic Res., 15: 385-391.
31.
Caipang CM, Takano T, Hirono I, Aoki T. (2006) Genetic vaccines protect red
seabream, Pagrus major, upon challenge with red seabream iridovirus (RSIV).
Fish Shellfish Immunol. 21: 130-138.
32. Somboonwiwat K, Supungul P,
Rimphanitchayakit V, Aoki T, Hirono I, Tassanakajon A. (2006) Differentially
Expressed Genes in Hemocytes of Vibrio
harveyi-challenged Shrimp Penaeus
monodon. J Biochem Mol Biol., 39: 26-36.
33. Byon JY, Ohira T, Hirono I, Aoki T. (2006)
Comparative immune responses in Japanese flounder, Paralichthys olivaceus after vaccination with viral hemorrhagic
septicemia virus (VHSV) recombinant glycoprotein and DNA vaccine using a
microarray analysis. Vaccine 24:921-930.
34. Byon JY, Takano T, Hirono I, Aoki T. (2006) Genome analysis of viral
hemorrhagic septicemia virus (VHSV) isolated from Japanese flounder, Paralichthys olivaceus in Japan.
Fisheries Science, 72:906-908.
35. Aoki T, Hirono I. (2006) Immune relevant genes of Japanese
flounder, Paralichthys olivaceus.
Comp. Biochem. Physiol. Part D, 1:115-121.
36. Santos MD, Yasuike M, Hirono I, Aoki T.
(2006) The granulocyte colony-stimulating factors (CSF3s) of fish and chicken.
Immunogenetics, 58:422-432.
37. Byon JY, Ohira T, Hirono I, Aoki T. (2005) Use of a cDNA
microarray to study immunity against viral hemorrhagic septicemia (VHS) in
Japanese flounder (Paralichthys olivaceus)
following DNA vaccination. Fish Shellfish Immunol., 18:135-147.
38. Yazawa, R., Hirono, I., Ohira, T. and Aoki, T. (2005) Functional
analysis of tumor necrosis factor gene promoter from Japanese flounder, Paralichthys olivaceus, using fish cell
lines. Dev. Comp. Immunol., 29: 73-81.
39. Park, C.-I., Hirono I, Aoki T. (2005) Molecular characterization
of the Japanese flounder, Paralichthys
olivaceus, CD3e and
evolution of the CD3 cluster. Dev. Comp. Immunol., 29:123-133.
40. Ooi EL, Ohira T, Hirono I, Taki T, Aoki T. (2005) Immunoanalysis
of antiviral Mx protein expression in Japanese flounder (Paralichthys olivaceus) cells. Dev. Comp. Immunol., 29:443-455.
41. Rattanachai A, Hirono I, Ohira T, Takahashi Y, Aoki T. (2005)
Peptidoglycan inducible expression of a serine proteinase homologue from kuruma
shrimp (Marsupenaeus japonicus). Fish
Shellfish Immunol., 18:39-48.
42. Caipang CM, Hirono I, Aoki T. (2005) Induction of antiviral state
in fish cells by Japanese flounder, Paralichthys
olivaceus, interferon regulatory factor-1. Fish Shellfish Immunol., 19:
79-91.
43. Kurobe T, Yasuike M, Kimura Y, Hirono I, Aoki T. (2005) Expression
profiling of immune-related genes from Japanese flounder Paralichthys olivaceus kidney cells using cDNA microarray. Dev.
Comp. Immunol., 29: 515-523.
44. Arma NR, Hirono I, Aoki T. (2005) Identification of genes
expressed in the liver of Japanese flounder (Paralichthys olivaceus) by expressed sequence tags. Fisheries
Science, 71: 504-518.
45. Emmadi D, Hirono I, Aoki T. (2005) cDNA microarray analysis of
interleukin-1b induced Japanese flounder Paralichthys
olivaceus kidney cells. Fisheries Science, 71: 519-530.
46. Shiina T, Dijkstra JM, Shimizu S, Watanabe A, Yanagiya K, Kiryu I,
Fujiwara A, Nishida-Umehara C, Kaba Y, Hirono I, Yoshiura Y, Aoki T, Inoko H,
Kulski JK, Ototake M. (2005) Interchromosomal duplication of major
histocompatibility complex class I regions in rainbow trout (Oncorhynchus mykiss), a species with a
presumably recent tetraploid ancestry. Immunogenetics, 56: 878-893.
47. Yazawa R, Hirono I, Ohira T, Aoki T. (2005) Induction of Japanese
flounder TNF promoter activity by lipopolysaccharide in zebrafish embryo. Mar.
Biotechnol., 7:231-235.
48. Dalla Valle L, Toffolo V, Vianello S, HironoI, Aoki T, Belvedere
P, Colombo L. (2005) Genomic organization of the CYP19b genes in the rainbow
trout (Oncorhynchus mykiss Walbaum). J
Steroid Biochem Mol Biol., 94:49-55.
49. Yazawa R, Hirono I, Aoki T. (2005) Characterization of promoter
activities of four different Japanese flounder promoters in transgenic zebrafish.
Marine Biotechnol., 7: 625-633.
50. Yazawa R, Hirono I, Yamamoto E, Aoki T. (2005) Gene transfer for
Japanese flounder fertilized eggs by particle gun bombardment. Fisheries
Science, 71: 869-874.
51. Park C-I, Hirono I, Aoki T. (2005) Characterization and expression
of a CD40 homologue gene in Japanese flounder Paralichthys olivaceus. Immunogenetics, 28: 1-8.
52.
Hirono I, Hwang JY, Ono Y, Kurobe T, Ohira T, Nozaki R, Aoki T. (2005) Two
different types of hepcidins from Japanese flounder Paralichthys olivaceus. FEBS J., 272: 5257-5264.
53. Arma
NR, Hirono I, Aoki T. (2005) Genes expressed in Japanese flounder (Paralichthys olivaceus) spleen: Analysis
of genes involved in immune function. Fisheries Science, 71: 1304-1323.
54. Yazawa R, Watanabe K, Koyama T, Ruangpan L, Tassanakajon A, Hirono
I, Aoki T. (2005) Development of gene transfer technology for black tiger
shrimp Penaeus monodon. J. Exp.
Zool., 303: 1104-1109.
55. Lua DT, Yasuike M, Hirono I, Aoki T. (2005)
Transcription program of red seabream iridovirus as revealed by DNA
microarrays. J. Virol., 79: 15151-15164.
56. Caipang CM, Haraguchi I, Ohira T, Hirono I, Aoki T. (2004) Rapid
detection of a fish iridovirus using loop-mediated isothermal amplification
(LAMP). J. Virol. Methods., 121:155-161.
57. Hwang JY, Ohira T, Hirono I, Aoki T. (2004) A pore-forming
protein, perforin, from a non-mammalian organism, Japanese flounder Paralichthys olivaceus. Immunogenetics, 56:360-367.
58. Srisapoome P, Ohira T, Hirono I, Aoki T. (2004) Genes of the constant
regions of functional immunoglobulin heavy chain of Japanese flounder, Paralichthys olivaceus. Immunogenetics, 56:292-300.
59. Rattanachai A, Hirono I, Ohira T, Takahashi Y, and Aoki T. (2004)
Cloning of kuruma prawn Marsupenaeus
japonicus crustin-like peptide cDNA and analysis of its expression.
Fisheries Science, 70: 765-771.
60. Takano T, Iwahori A, Hirono I, Aoki T. (2004) Development of a DNA
vaccine against hirame rhabdovirus and analysis of the expression of
immune-related genes after vaccination. Fish Shellfish Immunol., 17: 367-374.
61. Rattanachai A, Hirono I, Ohira T, Takahashi Y, and Aoki T. (2004) Molecular
cloning and expression analysis of alpha 2-macroglobulin in the kuruma shrimp, Marsupenaeus japonicus. Fish Shellfish
Immunol. 16:599-611.
62. Hirono I, Takami M, Miyata M, Miyazaki T, Han HJ, Takano T, Endo M,
Aoki T. (2004) Characterization of gene structure and expression of two
toll-like receptors from Japanese flounder, Paralichthys
olivaceus. Immunogenetics, 56:38-46.
63. Khattiya R, Ohira T, Hirono I, and Aoki T. (2004) Identification
of a novel Japanese flounder (Paralichthys
olivaceus) CC chemokine gene and an analysis of its function.
Immunogenetics, 55:763-769.
64. Hirono I, Yazawa R. Aoki T. (2004) Expressed sequence tag of Japanese
flounder (Paralichthys olivaceus)
skin cells. Fisheries Science, 70:195-197.
65. Srisapoome P, Ohira T, Hirono I, Aoki T. (2004) Cloning,
characterization and expression of cDNAs containing major histocompatibility
complex class I, IIa and IIb genes of Japanese flounder Paralichthys olivaceus. Fisheries Science, 70:264-276.
66. Arma NR, Hirono I, Aoki T. (2005) Characterization of expressed
genes in kidney cells of Japanese flounder Paralichthys
olivaceus following treatment with ConA/PMA and LPS. Fish Pathology, 39:
189-196.
67. Supungul P, Klinbunga S, Pichyangkura R, Hirono I, Aoki T,
Tassanakajon A. (2004) Antimicrobial peptides discovered in the black tiger
shrimp Penaeus monodon using the EST
approach. Dis Aquat Organ. 61:123-135.
68. Aoki T, Rattanachai A, Tomura N, Watanabe K, Itami T, Takahashi Y,
Ohira T, Hirono I. (2004) Molecular mechanisms of biodefense in kuruma shrimp.
Mar. Biotechnol., 6: 153-157.
69. Caipang CM, Hirono I, Ohira T, Aoki T. (2004) Possible role of
Mx, an interferon-inducible gene, in antiviral response in teleosts. Mar. Biotechnol., 6: 475-479.
70. Leelatanawit R, P, Klinbunga Puanglarp P, Tassanakajon A,
Jarayabhand P, Hirono I, Aoki T, Menasveta P. (2004) Isolation and
characterization of differentially expressed genes in ovaries and testes of the
giant tiger shrimp (Penaeus monodon).
Mar. Biotechnol., 6: 506-510.
著書
1. 海洋生物の機能（竹井祥邦編）「ヒラメの免疫関連遺伝子と耐病性」(青木 宙、廣野育生)、2005、東海大学出版会、pp.246-260.
2. Current Trends in the Study
of Bacterial and Viral Fish and Shrimp Diseases (Ed. by Leung Ka Yin),
Molecular Aspect of Fish and Marine Biology-Vol.3, 「Molecular Immunity in Fish-Pathogen
Interactions」 (Aoki T,
Hirono I.)、2004、World Scientific、 pp. 256-291.
学会発表
19年度
18年度
1. ペプチドグリカン投与クルマエビ血球で発現する遺伝子のマイクロアレイ解析、Fernand Fagutao・安池元重・近藤秀裕・廣野育生・青木宙・高橋幸則、平成18年度日本水産学会大会 平成18年3月（高知大学）
2. ヒラメのTおよびBリンパ球で発現する免疫グロブリンスーパーファミリーNITRについて、Praprudee Piyaviriyakul・近藤秀裕・廣野育生・青木 宙、平成18年度日本水産学会大会 平成18年3月（高知大学）
3. ヒラメToll-like receptor 9の体系的研究、高野倫一・近藤秀裕・廣野育生・青木 宙、第9回マリンバイオテクノロジー学会 平成18年5月（東京海洋大学）
4. ヒラメ肝臓ESTデータを利用した血漿タンパク質遺伝子の同定、近藤秀裕・安池元重・廣野育生・青木宙（海洋大）、第9回マリンバイオテクノロジー学会 平成18年5月（東京海洋大学）
5. A novel immune-type receptor of Japanese
flounder (Paralichthys olivaceus): cloning and characterization、Praprudee Piyaviriyakul・近藤秀裕・廣野育生・青木 宙第9回マリンバイオテクノロジー学会 平成18年5月（東京海洋大学）
6. Gene expression Profile of PG timulated
heamocytes of Kuruma Shrimp、Fernand
Fagutao・安池元重・近藤秀裕・廣野育生・青木宙・高橋幸則第9回マリンバイオテクノロジー学会 平成18年5月（東京海洋大学）
7. A Study on Pentraxin from Japanese flounder in
comparison with other teleost species、Gung Andrew・近藤秀裕・廣野育生・青木宙、第9回マリンバイオテクノロジー学会 平成18年5月（東京海洋大学）
8. ESTs analysis of immune related genes of red
sea bream (Pagrus major) kidney cells stimulated with ConA/LPS、Damri Darawiroj・近藤秀裕・廣野育生・青木 宙、第9回マリンバイオテクノロジー学会 平成18年5月（東京海洋大学）
9. Identification of two novel Japanese flounder
Paralichthys olivaceus CC chemokine genes、Hyun-Jeong Kim・近藤秀裕・廣野育生・青木 宙、第9回マリンバイオテクノロジー学会 平成18年5月（東京海洋大学）
10. MOLECULAR AND FUNCTIONAL ANALYSYS of Interferon-stimulated
gene 15 (ISG15) From the Japanese flounder, Paralichthys
olivaceus、Motoshige
Yasuike, Yasuhito Ono, Hidehiro Kondo, Ikuo Hirono and Aoki Takashi、第10回国際比較免疫学会 平成18年7月（チャールストン）
11. Characterization of regulation of gene
expression of Japanese flounder TNF gene、Ikuo Hirono, Ryosuke Yazawa, Hidehiro Kondo
and Takashi Aoki、第10回国際比較免疫学会 平成18年7月（チャールストン）
12. GENE EXPRESSION PROFILE OF KURUMA SHRIMP, Marsupenaeus japonicus, HAEMOCYTES AFTER
PEPTIDOGLYCAN STIMULATION、Takashi
Aoki, Fernand Fagutao, Motoshige Yasuike, Hidehiro Kondo, Ikuo Hirono, Yukinori
Takahashi, and Anchalee Tassanakajon、第10回国際比較免疫学会 平成18年7月（チャールストン）
13. DNA vaccination and gene expression profile in
fish、Takashi Aoki, Christopher M. A. Caipang and
Ikuo Hirono、第10回国際比較免疫学会 平成18年7月（チャールストン）
14. ANALYSIS OF THE GENE EXPRESSION PROFILE OF
KURUMA SHRIMP Marsupenaeus japonicus IN RESPONSE TO PEPTIDOGLYCAN STIMULATION、Fernand Fagutao, Motoshige Yasuike, Hidehiro
Kondo, Ikuo Hirono, Yukinori Takahashi, Takashi Aoki、第5回水棲生物健康に関する国際会議 平成18年9月（サンフランシスコ）
15. Acute phase proteins gene expression and
their transcriptional control in liver of Japanese flounder, Paralichthys olivaceus、Andrew Gung Yeu Tzeh, Hidehiro Kondo, Ryosuke
Yazawa, Motoshige Yasuike, Ikuo Hirono, Takashi Aoki第5回水棲生物健康に関する国際会議 平成18年9月（サンフランシスコ）
16. IDENTIFICATION OF TWO NOVEL JAPANESE
FLOUNDER Paralichthys olivaceus CC
CHEMOKINE GENES、Hyun-Jeong
Kim, Hidehiro Kondo, Ikuo Hirono, Takashi Aoki、第5回水棲生物健康に関する国際会議 平成18年9月（サンフランシスコ）
17. A NOVEL IMMUNE-TYPE RECEPTOR OF JAPANESE
FLOUNDER, Paralichthys olivaceus:
MOLECULAR CLONING AND CHARACTERIZATION、Prapruddee Piyaviriyakul, Hidehiro Kondo, Ikuo
Hirono, Takashi Aoki、第5回水棲生物健康に関する国際会議 平成18年9月（サンフランシスコ）
18. Molecular immune- and biodefense-mechanism
of penaid shrimp、Takashi
Aoki, Hidehiro Kondo, Ikuo Hirono、第5回水棲生物健康に関する国際会議 平成18年9月（サンフランシスコ）
19. MOLECULAR CLONING AND CHARACTERIZATION OF TWO
NOVEL JAPANESE FLOUNDER Paralichthys
olivaceus CC CHEMOKINE GENES、Hyun-Jeong Kim*, Hidehiro Kondo, Ikuo Hirono,
Takashi Aoki、アジア太平洋アクアゲノム研究会議 平成18年11月（ハワイ）
20. Transcriptional control of acute phase protein
in Japanese flounder, Paralichthys
olivaceus、Hidehiro
Kondo, Andrew Gung Yeu Tzeh, Ryosuke Yazawa, Motoshige Yasuike, Ikuo Hirono,
Takashi Aoki、アジア太平洋アクアゲノム研究会議 平成18年11月（ハワイ）
21. MICROARRAY ANALYSIS OF GENE EXPRESSION IN
KURUMA SHRIMP UPON PEPTIDOGLYCAN STIMULATION、Takashi Aoki, Fernand
Fagutao, Motoshige Yasuike, Hidehiro Kondo, Ikuo Hirono, Yukinori Takahashi、アジア太平洋アクアゲノム研究会議 平成18年11月（ハワイ）
17年度
1. ヒラメのIL1および２種類のケモカインのアジュバント効果について、Emmadi Dhanwanthari・廣野育生・青木宙、平成17年度日本水産学会春季大会、平成17年4月（東京海洋大学）
2.
Expression profile of Japanese flounder (Paralichthys olivaceus) head kidney cells using poly I:C as a
stimulator、Praprudee
Piyaviriyakul・廣野育生・青木宙、平成17年度日本水産学会春季大会、平成17年4月（東京海洋大学）
3. DNAワクチン接種ヒラメにおいて誘導されたユビキチン様タンパク質遺伝子の構造および発現解析、安池元重・廣野育生・青木宙、平成17年度日本水産学会春季大会、平成17年4月（東京海洋大学）
4. ポリICで刺激したヒラメ脳細胞における遺伝子発現プロファイル、原口郁美・廣野育生・青木宙、平成17年度日本水産学会春季大会、平成17年4月（東京海洋大学）
5. ヒラメEST解析より得られた新規CXCケモカイン遺伝子の構造および発現解析安池元重・近藤秀裕・廣野育生・青木 宙、平成17年度日本魚病学会大会、平成17年9月（三重大学）
6. ブリおよびマダイの腎臓細胞で発現している遺伝子のEST解析、Damri Darawiroj・佐藤健朗・野崎玲子・廣野育生・青木 宙、第8回マリンバイオテクノロジー学会 平成17年5月（熊本県立大学）
7. Application of DNA microarray technology for the study of resistance
to infectious disease in Japanese flounder (Paralichthys
olivaceus)、M. Yasuike,
T. Takano, I. Hirono and T.
Aoki、第12回ヨーロッパ魚病学会 平成17年9月（コペンハーゲン）
8. Immune-related genes profile obtained from
Red seabream and Yellowtail kidney cells by using EST analysis、D. Darawiroj, I. Hirono and T. Aoki、第6回アジア水産学会魚病セクションシンポジウム、平成17年10月（スリランカ）
9. Study on poly I:C stimulation in Japanese
flounder (Paralichthys olivaceus)
head kidney cells through ESTs analysis、P. Piyaviriyakul, I. Hirono and T. Aoki、第6回アジア水産学会魚病セクションシンポジウム、平成17年10月（スリランカ）
10. Identification
of immune-related genes from Japanese flounder brain cells stimulated with poly
I:C by gene expression profiling、I. Haraguchi, H. Kondo, I. Hirono and T. Aoki、第6回アジア水産学会魚病セクションシンポジウム、平成17年10月（スリランカ）
11. Transcriptome
Study of Japanese Flounder, Paralichthys
olivaceus using 3rd version of cDNA microarray、I. Hirono, M. Yasuike, I. Haraguchi, T.
Hirota, H. Kondo and T. Aoki、第14回国際植物動物ゲノム会議 平成18年1月（サンディエゴ）
16年度
1. マイクロアレイを用いたヒラメの病原微生物感染応答遺伝子解析、安池元重・大平剛・廣野育生・青木宙、平成16年度日本水産学会春季大会、平成16年4月（鹿児島大学）
2. DNA microarray analysis of biodefence-related
gene expression characteristics in HINAE cell lines in response to RSIV
infection、Dang Lua
Thi・大平剛・廣野育生・青木宙、平成16年度日本魚病学会大会、平成16年9月（北海道）
3. ポリI:Cで刺激したヒラメの腎臓および脳において発現する遺伝子のEST解析、廣野育生・Praprudee Piyankul・原口郁美・青木 宙、第29回国際動物遺伝育種会議、平成16年9月（明治大学、東京）
4. Gene expression profiling of different group
of Japanese flounder by using DNA microarray analysis、Motoshige Yasuike, Tomokazu Takano,
Tsuyoshi Ohira, Ikuo Hirono and Takashi Aoki、第77回日本生化学会大会、平成16年10月（横浜）
5. Gene expression profiles in different groups
of Japanese flounder with distinct susceptibility to infectious disease、Motoshige Yasuike, Tomokazu Takano, Tsuyoshi
Ohira, Ikuo Hirono and Takashi Aoki、平成16年度日本水産学会関東支部大会、平成16年11月（東京大学）
6. ポリ I:C で刺激したヒラメの脳において発現する遺伝子のEST解析、原口郁実・廣野育生・青木宙、平成16年度日本水産学会関東支部大会、平成16年11月（東京大学）
7. ESTs analysis of Japanese flounder (Paralichthys olivaceus) head kidney
cells stimulated with poly I:C、Piyaviriyakul
Prapruddee, Ikuo Hirono and Takashi Aoki、平成16年度日本水産学会関東支部大会、平成16年11月（東京大学）
8. The adjuvant effects of IL-1, CXC chemokine
and MIP1a in DNA vaccination against viral haemorrhagic septicemia virus in
Japanese flounder、Emmadi
Dhanwanthari, Ikuo Hirono and Takashi Aoki、平成16年度日本水産学会関東支部大会、平成16年11月（東京大学）
9. EST-based identification of genes expressed in
the liver of adult Japanese flounder (Paralichthys
olivaceus)、Nur
Rahmawaty Arma, Ikuo Hirono and Takashi Aoki、平成16年度日本水産学会関東支部大会、平成16年11月（東京大学）
10. ヒラメcDNAマイクロアレイの魚類凡用性の検討、安池元重・廣野育生・青木宙、平成16年度日本水産学会関東支部大会、平成16年11月（東京大学）
11. Potential use of a cDNA microarray in the
study of Viral Hemorrhagic Septicemia Virus (VHSV) infection in fish、Ju Yong Byon, Ikuo Hirono and Takashi Aoki、DNAワクチン2004、平成16年11月（モナコ）
12. Application of DNA microarray for
characterization of the immune system during DNA vaccination in fish、Motoshige Yasuike, Ikuo Hirono and Takashi
Aoki、DNAワクチン2004、平成16年11月（モナコ）
15. DEVELOPMENT OF DNA VACCINES FOR JAPANESE
FLOUNDER Paralichthys olivaceus AND
ANALYSIS OF THE IMMUNE RESPONSE AFTER VACCINATION、Tomokazu Takano, Motoshige Yasuike, Ju Yong
Byon, Ikuo Hirono, Takashi Aoki、第7回アジア水産学会、平成16年11～12月（マレーシア）
16. KINETIC
TRANSCRIPTION PROFILE OF RED SEA BREAM IRIDOVIRUS AS CHARACTERIZED BY DNA
MICROARRAY、Dang Thi Lua
, Ikuo Hirono and Takashi Aoki、第7回アジア水産学会、平成16年11～12月（マレーシア）
17. マダイイリドウイルス全遺伝子の発現プロファイリング、Dang Lua Thi、廣野育生、大平剛、青木宙（東京海洋大学）、第7回マリンバイオテクノロジー学会、平成16年6月（北海道大学）
18. Gene expression profiling of red seabream
iridovirus genes using microarray、Dang Lua Thi・大平剛・廣野育生・青木宙、第６回国際下等脊椎動物ウイルス学会、平成16年9月（北海道）
15年度
1. Takashi Aoki, Motoshige Yasuike, and Ikuo
Hirono：Gene
expression profiling of Japanese flounder by microarrays: a powerful tool for
directed identification of important genes for selective breeding in
aquaculture. Genetics
in Aquaculture VIII、平成15年11月（チリ）
2. 卞 珠瑩 ・高野倫一 ・大平 剛 ・廣野育生 ・青木 宙：ヒラメVHSウイルスに対するDNAワクチンチンの開発. 平成15年度日本魚病学会大会、平成15年10月、（下関）
3. Ikuo Hirono and Takashi Aoki：Functional cDNA microarray analysis of
Japanese flounder, Paralichthys olivaceus
for characterization of the immune system. Marine Biotechnology Conference 2003、平成15年9月、（千葉）
4. Motoshige Yasuike, Ikuo Hirono, and Takashi
Aoki：Differential gene expression profile of Japanese
flounder (Paralichthys olvivaceus) in
response to DNA vaccine utilizing microarray analysis. Marine Biotechnology Conference 2003、平成15年9月、（千葉）
5. Motoshige Yasuike, Ikuo Hirono, and Takashi
Aoki：Microarray analysis of gene expression during
DNA vaccination in Japanese flounder (Paralichthys
olivaceus). The 2nd
International Symposium on Aquatic Genomics、平成15年9月、（東京）