本文へスキップ

東京海洋大学 吉崎研究室

〒108-8477 東京都港区港南4-5-7

発表論文Publications

主要論文


2019年

雨澤孝太朗,尾崎雄一,矢澤良輔,竹内裕,風藤行紀,吉崎悟朗
粗精製生殖腺刺激ホルモン放出ホルモンアナログ(GnRHa)の経口投与によるゴマサバの初期卵形成促進技術の開発
日本水産学会誌 86(2), 83-90
Link to:日本水産学会誌

Zoran Marinovic, Qian Li, Jelena Lujic, Yoshiko Iwasaki, Zsolt Csenki, Bela Urbanyi, Goro Yoshizaki, Akos Horvath
Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation
Sci Rep 9, 13861
Link to: Sci Rep

Kensuke Ichida, Makoto Hayashi, Misako Miwa, Ryota Kitada, Momo Takahashi, Ryo hujihara, Surintorn Boonanuntanasarn, Goro Yoshizaki
Enrichment transplantable germ cells in salmonids using a novel monoclonal antibody by magnetic activated cell sorting
Mol Reprod Dev 86(12)
Link to: Mol Reprod Dev

Makoto Hayashi, Kensuke Ichida, Sakiko Sadaie, Misako Miwa, Ryo Fujiwara, Yasuhiko Nagasaka, Goro Yoshizaki
Establishment of novel monoclonal antibodies for identification of type A spermatogonia in teleosts.
Biol Reprod 101(2), 478-491
Link to: Biol Reprod

Kensuke Ichida, Wataru Kawamura, Misako Miwa, Yoshiko Iwasaki, Tsubasa Kubokawa, Makoto Hayashi, Ryosuke Yazawa, Goro Yoshizaki.
Specific visualization of live type A spermatogonia of Pacific bluefin tuna using fluorescent dye-conjugated antibodies.
Biol Reprod 100(6), 1637-1647
Link to: BOR

Rungsun Duangkaew, Ayara Jangprai, Kensuke Ichida, Goro Yoshizaki, Surintorn Boonarnuntanasarn.
Characterization and expression of a vasa homolog in the gonads and primordial germ cells of the striped catfish (Pangasianodon hypophthalmus).
Theriogenology 131, 61-71
Link to: Theriogenology

Asano Ishikawa, Naoki Kabeya, Koki Ikeya, Ryo Kakioka, Jennifer N. Cech, Naoki Osada, Miguel C. Leal, Jun Inoue, Manabu Kume, Atsushi Toyoda, Ayumi Tezuka, Atsushi J. Nagano, Yo Y. Yamasaki, Yuto Suzuki, Tomoyuki Kokita, Hiroshi Takahashi, Kay Lucek, Carsten J. Schubert, Blake Matthews, Catherine L. Peichel, Ole Seehausen, Goro Yoshizaki, Jun Kitano.
A key metabolic gene for recurrent freshwater colonization and radiation in fishes
Science 364, 886-889
Link to: Science

Goro Yoshizaki and Ryosuke Yazawa.
Application of surrogate broodstock technology in aquaculture.
Fisheries Science 85, 429-437
Link to: Fisheries Science

Ryosuke Yazawa, Yutaka Takeuchi, Yuri Machida, Kotaro Amezawa, Naoki Kabeya, Reoto Tani, Wataru Kawamura, Goro Yoshizaki.
Production of triploid eastern little tuna, Euthynnus affinis (Cantor, 1849).
Aquaculture Research 50,1422-1430
Link to: Aquaculture Research

Ana CarinaVasconcelos, Danilo Pedro Streit Jr, Anna Octavera, Misako Miwa, Naoki Kabeya and Goro Yoshizaki.
The germ cell marker dead end reveals alternatively spliced transcripts with dissimilar expression.
Sci Rep 9, 2407
Link to: Sci Rep

2018年

Ana Carina Nogueira Vasconcelos, Danilo Pedro Streit Jr, Anna Octavera, Misako Miwa, Naoki Kabeya, Raycon Roberto Freitas Garcia, Daniel Antonio Rotili, Goro Yoshizaki.
Isolation and characterization of a germ cell marker in teleost fish Colossoma macropomum.
Gene 683, 54-60
Link to: Gene

Anna Octavera and Goro Yoshizaki.
Production of donor-derived offspring by allogeneic transplantation of spermatogonia in Chinese rosy bitterling.
Biol Reprd 100, 1108-1117
Link to: BOR

Sylvain Bertho, Amaury Herpin, Adele Branthonne, Elodie Jouanno, Ayaka Yano, Barbara Nicol, Thomas Muller, Maelle Pannetier, Eric Pailhoux, Misako Miwa, Goro Yoshizaki, Manfred Schartl, Yann Guiguen.
The unusual rainbow trout sex determination gene hijacked the canonical vertebrate gonadal differentiation pathway.
PNAS 115, 12781-12786
Link to: PNAS

Hiroyuki Yoshikawa, Dongdong Xu, Yasuko Ino, Tasuku Yoshino, Takao Hayashida, Junjie Wang, Ryosuke Yazawa, Goro Yoshizaki and Yutaka Takeuchi.
Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells.
Genetics 209, 507-521
Link to: Genetics

Kazue Nagasawa, Mariko Ishida, Anna Octavera, Kazunari Kusano, Fumi Kezuka, Takeshi Kitano, Yasutoshi Yoshiura, Goro Yoshizaki.
Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation.
Biol Reprod. 100, 535-546
Link to: BOR

Tomotsune Ameku, Yuto Yoshinari, Micheal J. Texada, Shu Kondo, Kotaro Amezawa, Goro Yoshizaki, Yuko Shimada-Niwa, Ryusuke Niwa.
Midgut-derived neuropeptide F controls germline stem cell proliferation in a mating-dependent manner.
Plos Biology 24
Link to: Plos Biology

Xuanchen Zhang, Masato Endo, Takashi Sakamoto, Reiko Fuseya, Goro Yoshizaki, Toshio Takeuchi.
Studies on kuruma shrimp culture in recirculating aquaculture system with artificial ecosystem.
Aquaculture 484, 191-196
Link to: Aquaculture

Goro Yoshizaki and Seungki Lee.
Production of live fish derived from frozen germ cells via germ cell transplantation.
Stem Cell Research 29, 103-110
Link to: Stem cell Research

Kotaro Amezawa, Ryosuke Yazawa, Yutaka Takeuchi, Goro Yoshizaki.
Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of crude gonadotropin-releasing hormone analogue.
Fisheries Science 84, 495-504
Link to: Fisheries Science

Makoto Hayashi, Daika Sakuma, Goro Yoshizaki.
Production of functional sperm by subcutaneous auto-grafting of immature testes in rainbow trout
Mol Reprod Dev. 85, 155-162
Link to: Mol Reprod Dev.

2017年

Masaomi Hamasaki, Yutaka Takeuchi, Ryosuke Yazawa, Souta Yoshikawa, Kazushi Kadomura, Toshiyuki Yamada, Kadoo Miyaki, Kiyoshi Kikuchi and Goro Yoshizaki.
Production of tiger puffer Takifugu rubripes offspring from triploid grass puffer Takifugu niphobles parents.
Marine Biotechnology 19, 579-591
Link to: Marine Biotechnology

Naoki Kabeya, Mizumo Chiba, Yutaka Haga, Shuichi Satoh, Goro Yoshizaki
Cloning and functional characterization of fads2 desaturase 1 and elovl5 elongase from Japanese flounder Paralichthys olivaceus
Cpmparative Biochemistry and Physiology B 214, 36-46
Link to: Comparative Biochemistry and Physiology B

Li Q, Fujii W, Naito K, Yoshizaki G.
Application of dead end-knockout zebrafish as recipients of germ cell transplantation.
Mol Reprod Dev. 84, 1100-1111
Link to: Mol Reprod Dev.

Kensuke Ichida, Kazuyoshi Kise, Tetsuro Morita, Ryosuke Yazawa, Yutaka Takeuchi, Goro Yoshizaki
Flow-cytometric enrichment of Pacific bluefin tuna type A spermatogonia based on light-scattering properties.
Theriogenology 101, 91-98
Link to: Theriogenology

Mana Sato, Makoto Hayashi, Goro Yoshizaki
Stem cell activity of type A spermatogonia is seasonally regulated in rainbow trout.
Biol Reprod. 96: 6, 1303-1316
Link to: BOR

Seki S, Kusano K, Lee S, Iwasaki Y, Yagisawa M,Ishida M, Hiratsuka T, Sasado T, Naruse K, Yoshizaki G
Production of the medaka derived from vitrified whole testes by germ cell transplantation.
Scientific Reports 7, 43185
Link to: Scientific Reports Journal

2016年

R. Yazawa, Y. Takeuchi, K. Amezawa, K. Sato, G. Iwata, N. Kabeya, G. Yoshizaki
GnRHa-induced spawning of the Eastern little tuna (Euthynnus affinis) in a 70-m3 land-based tank.
Aquaculture 442, 58-68
Link to: Aquaculture

N. Kabeya, Y. Takeuchi, R. Yazawa, Y. Haga, S. Satoh, G. Yoshizaki
Transgenic modification of the n-3 HUFA biosynthetic pathway in nibe croaker larvae: improved DPA (docosapentaenoic acid; 22:5n-3) production.
Aquaculture Nutrition 22, 472-478
Link to: Aquaculture Nutrition

Lee S, Katayama N, Yoshizaki G.
Generation of juvenile rainbow trout derived from cryopreserved whole ovaries by intraperitoneal transplantation of ovarian germ cells.
Biochem Biophys Res Commun. 478, 1478-1483
Link to: Biochem Biophys Res Commun.

Lee S, Iwasaki Y, Yoshizaki G.
Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids.
Cryobiology 73, 286-290
Link to: Cryobiology

Boonanuntanasarn S, Bunlipatanon P, Ichida K, Yoohat K, Mengyu O, Detsathit S, Yazawa R, Yoshizaki G.
Characterization of a vasa homolog in the brown-marbled grouper (Epinephelus fuscoguttatus) and its expression in gonad and germ cells during larval development.
Fish Physiol Biochem. 42, 1621-1636
Link to: Fish Physiol Biochem.

Katayama N, Kume S, Hattori-Ihara S, Sadaie S, Hayashi M, Yoshizaki G.
Germ Cell-Specific Excision of loxP-Flanked Transgenes in Rainbow Trout Oncorhynchus mykiss.
Biol Reprod. 94: 79, 1-11
Link to: BOR

Yoshizaki G, Takashiba K, Shimamori S, Fujinuma K, Shikina S, Okutsu T, Kume S, Hayashi M.
Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation.
Mol Reprod Dev. 83, 298-311
Link to: Mol Reprod Dev.

Lee S, Yoshizaki G.
Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok).
Cryobiology 72, 165-168
Link to:Cryobiology

Hiroyuki Yoshikawa, Yutaka Takeuchi, Yasuko Ino, Junjie Wang, Gaku Iwata, Naoki Kabeya, Ryosuke Yazawa, Goro Yoshizaki
Efficient production of donor-derived gametes from triploid recipients following intra-peritoneal germ cell transplantation into a marine teleost, Nibe croaker (Nibea mitsukurii).
Aquaculture 478, 35-47
Link to: Aquaculture

2015年

Bar I, Dutney L, Lee P, Yazawa R, Yoshizaki G, Takeuchi Y, Cummins S, Elizur A.
Small-scale capture, transport and tank adaptation of live, medium-sized Scombrids using "Tuna Tubes".
Springerplus
Link to: Springerplus

Tomonori Kuwada, Testsuya Tokuhara, Munetaka Shimizu, Goro Yoshizaki
Body size is the primary regulator affecting commencement of smolting in amago salmon Oncorhynchus masou ishikawae.
Fisheries Science 82, 59-71
Link to: Fisheries Science

Seungki Lee, Shinsuke Seki, Naoto Katayama & Goro Yoshizaki
Production of viable trout offspring derived from frozen whole fish.
Link to: Scientific reports

N Kabeya, Y Yamamoto, SF Cummins, A Elizur, R Yazawa, Y Takeuchi, Y Haga, S Satoh, G Yoshizaki
Polyunsaturated fatty acid metabolism in a marine teleost, Nibe croaker Nibea mitsukurii: Functional characterization of Fads2 desaturase and Elovl5 and Elovl4 elongases.
Comparative Biochemistry and Physiology Part B:
Biochemistry and Molecular Biology 188, 37-45
Link to: CBP Part B: Biochemistry and Molecular Biology

Tetsuro Morita, Kagayaki Morishima, Misako Miwa, Naoki Kumakura, Satomi Kudo, Kensuke Ichida, Toru Mitsuboshi, Yutaka Takeuchi, and Goro Yoshizaki
Functional sperm of the yellowtail (Seriola quinqueradiata) were produced in the small-bodied surrogate, jack mackerel (Trachurus japonicus)
Marine Biotechnology 17, 644-654
Link to: Marine Biotechnology

Ido Bar, Andre, Smith, Erin Bubner, Goro Yoshizaki, Yutaka Takeuchi, Ryosuke Yazawa, Ben Nan Chen, Scott Cummins, Abigail Elizur
Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of Southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation.
Reproduction,Fertility and Development 28, 2051-2064
Link to: Reproduction, Fertility and Development

Ryosuke Yazawa, Yutaka Takeuchi, Kenta Satoh, Yuri Machida, Kotaro Amezawa, Naoki Kabeya, Yukinori Shimada, Goro Yoshizaki
Eastern little tuna, Euthynnus affinis (Cantor, 1849) mature and reproduce within 1 year of rearing in land-based tanks.
Aquaculture Research 47, 3800-3810
Link to: Aquaculture Research

T. Okutsu, S. Shikina, T. Sakamoto, M. Mochizuki, and *G. Yoshizaki:
Successful production of functional Y eggs derived from spermatogonia transplanted into female recipients and subsequent production of YY supermales in rainbowtrout, Oncorhynchus mykiss.
Aquaculture 446, 298-302
Link to: Aquaculture

JA. Fernandez, EJ. Bubner, Y. Takeuchi, *G. Yoshizaki, T. Wang, SF. Cummins, A. Elizur
Primordial germ cell migration in the yellowtail kingfish (Seriola lalandi) and identification of stromal cell-derived factor 1.
Gen Comp Endocrinol. 213, 16-23
Link to: Gen Comp Endocrinol.

R. Yazawa, Y. Takeuchi, K. Amezawa, K. Sato, G. Iwata, N. Kabeya, *G. Yoshizaki
GnRHa-induced spawning of the Eastern little tuna (Euthynnus affinis) in a 70-m3 land-based tank.
Aquaculture 442, 58-68
Link to: Aquaculture

S. Boonanuntanasarn, A. Jangprai, *G. Yoshizaki
Characterization of proopiomelanocortin in the snakeskin gourami (Trichopodus pectoralis) and its expression in relation to food intake.
Domest Anim Endocrinol. 50, 1-13
Link to: Domest Anim Endocrinol.

N Kabeya, Y Takeuchi, R Yazawa, Y Haga, S Satoh, G Yoshizaki
Transgenic modification of the n-3 HUFA biosynthetic pathway in nibe croaker larvae: improved DPA (docosapentaenoic acid; 22:5n-3) production.
Aquaculture Nutrition 22, 472-478
Link to: Aquaculture Nutrition

2014年
S Kume, N Katayama, K Ichida, S Hattori-Ihara, K Nagasawa, G Yoshizaki
Transgene manipulation in rainbow trout using Cre recombinase.
Fisheries Science
Link to: PubMed

M Hayashi, M Sato, Y Nagasaka, S Sadaie, S Kobayashi, G Yoshizaki
Enrichment of Spermatogonial Stem Cells Using Side Population in Teleost.
Biol Reprod.
Link to: Journal

S. Nakajima, M. Hayashi, T. Kouguchi, K. Yamaguchi, M. Miwa, G. Yoshizaki
Expression patterns of gdnf and gfra1 in rainbow trout testis
Gene Expression Patterns
Link to: PubMed Journal

W. Makkapan, G. Yoshizaki, M. Tashiro, W. Chotigeat
Expression profile of ribosomal protein L10a throughout gonadal development
Fish Physiology and Biochemistry
Link to:PubMed Journal

M. Sato, T. Morita, N. Katayama, G. Yoshizaki
Production of genetically diversified fish seeds using spermatogonial transplantation
Aquaculture 422-423, 218-224
Link to: PubMed Journal

N. Kabeya, Y. Takeuchi, Y. Yamamoto, R. Yazawa, Y. Haga, S. Satoh, G. Yoshizaki.
Modification of the n-3 HUFA biosynthetic pathway by transgenesis in a marine teleost, nibe croaker.
Journal of Biotechnology 172,46-54
Link to: PubMed Journal

2013年
R. Farlora, S. Hattori-Ihara, Y. Takeuchi, M. Hayashi, A. Octavera, Alimuddin, G. Yoshizaki.
Intraperitoneal germ cell transplantation in the nile tilapia oreochromis niloticus.
Marine Biotechnology in press.
Link to: PubMed Journal

R. Yazawa, Y. Takeuchi, T. Morita, M. Ishida, G. Yoshizaki.
The pacific bluefin tuna (Thunnus orientalis) dead end gene is suitable as a specific molecular marker of type A spermatogonia.
Molecular Reproduction and Development 871-880.
Link to: PubMed Journal

S. Shikina, K. Nagasawa, M. Hayashi, M. Furuya, Y. Iwasaki, and G. Yoshizaki.
Short-term in vitro culturing improves transplantability of type A spermatogonia in rainbow trout (Oncorhynchus mykiss).
Molecular Reproduction and Development 80, 763-773.
Link to: PubMed Journal

S. Lee, Y. Iwasaki, S. Shikina, G. Yoshizaki.
Generation of functional eggs and sperm from cryopreserved whole testes.
Proceedings of the National Academy of Sciencesof the United States of America 110, 1640-1645.
Link to: PubMed Journal

M. Hamasaki, Y. Takeuchi, K. Miyaki, G. Yoshizaki.
Gonadal development and fertility of triploid grass puffer Takifugu niphobles induced by cold shock treatment.
Marine Biotechnology 15, 133-144.
Link to:PubMed Journal

K. Nagasawa, JM. Fernandes, G. Yoshizaki, M. Miwa, I. Babiak.
Identification and migration of primordial germ cells in Atlantic salmon, Salmo salar: Characterization of vasa, dead end, and lymphocyte antigen 75 genes.
Molecular Reproduction and Development 80, 118-131.
Link to: PubMed Journal

SMSN. Lacerda, GMJ. Costa, PHA. Campos-Junior, TM. Segatelli, R. Yazawa, Y. Takeuchi, T. Morita, G. Yoshizaki, LR. Franca.
Germ Cell Transplantation as a Potential Biotechnological Approach to Fish Reproduction
Fish Physiology and Biochemistry 39, 3-11.
Link to: PubMed Journal

2012年
M. Hayashi, M. Sato, Y. Iwasaki, M. Terasawa, M. Tashiro, S. Yokoyama, N. Katayama, S. Sadaie, M. Miwa,
G. Yoshizaki.
Combining next-generation sequencing with microarray for transcriptome analysis in rainbow trout gonads.
Mol. Reprod. Dev. 79, 870-878. 2012.
=>Link to: PubMed Journal

T. Morita, N. Kumakura, K. Morishima, T. Mitsuboshi, M. Ishida, T. Hara, S. Kudo, M. Miwa, S. Ihara, K. Higuchi, Y. Takeuchi, G. Yoshizaki.
Production of donor-derived offspring by allogeneic transplantation of spermatogonia in the yellowtail (Seriola quinqueradiata).
Biol Reprod. 86(6):176. 2012.
=>Link to: PubMed Journal

G. Yoshizaki, T. Okutsu, T. Morita, M. Terasawa, R. Yazawa, Y. Takeuchi.
Biological characteristics of fish germ cells and their application to developmental biotechnology.
Reproduction in Domestic Animals 47, Suppl 4, 187-192. 2012.
=>Link to: PubMed Journal

K. Kise, H. Yoshikawa, M. Sato, M. Tashiro, R. Yazawa, Y. Takeuchi, G. Yoshizaki.
Flow-cytometric isolation and enrichment of teleost type-A spermatogonia based on light-scattering properties.
Biol. Reprod. 86(4):107.
=>Link to: PubMed Journal

K. Nagasawa, M. Miwa, R. Yazawa, T. Morita, Y. Takeuchi, G. Yoshizaki.
Characterization of lymphocyte antigen 75 (Ly75/CD205) as a potential cell-surface marker on spermatogonia in Pacific bluefin tuna Thunnus orientalis.
Fish. Sci. 78, 791-800. 2012.
=>Link to: PubMed Journal

K. Tsukamoto, F. Miura, N.T. Fujito, G. Yoshizaki, M. Nonaka.
Long-lived dichotomous lineages of the proteasome subunit beta type 8 (PSMB8) gene surviving more than 500 million years as alleles or paralogs.
Molecular Biology and Evolution 29, 3071-3079. 2012
=>Link to: PubMed Journal

H. Endo, T. Muramatsu, G. Yoshizaki, H. Ren, H. Ohnuki.
Development of a label-free immunosensor system for detecting oocyte maturation-inducing hormone in fish.
Fish. Sci. 78, 391-398. 2012.
=>Link to: PubMed Journal

S. Booanuntanasarn, A. Jangprai, G. Yoshizaki
Characterization of neuropeptide Y in snakeskin gourami and the change in its expression due to feeding status and melanocortin 4 receptor expression.
Gen. Comp. Endocrinol. 179, 184-195. 2012.
=>Link to: PubMed Journal

N. Phumyu, S. Boonanuntanasarn, A. Jangpria, G. Yoshizaki, U. Na-Nakorn.
Pubertal effects of 17α-methyltestosterone on GH-IGF-related genes of the hypothalamic-pituitary-liver-gonadal axis and other biological parameters in male, female and sex-reversed Nile tilapia.
Gen. Comp. Endocrinol. 177, 278-292. 2012.
=>Link to: PubMed Journal

J. Aizen, M. Kobayashi, I. Selicharova, Y.C. Sohn, G. Yoshizaki, B. Levavi-Sivan.
Steroidogenic response of carp ovaries to piscine FSH and LH depends on the reproductive phase.
Gen. Comp. Endocrinol. 178, 28-36. 2012
=>Link to: PubMed Journal


2011年

J.J. Nagler, T. Cavileer, S. Hunter, R. Drew, T. Okutsu, T. Sakamoto, G. Yoshizaki. 2011.
Non-sex specific genes associated with the secondary mitotic period of primordial germ cell proliferation in the gonads of embryonic rainbow trout (Oncorhynchus mykiss).
Mol Reprod Dev. 78, 181-187.
=>Link to: PubMed Journal

K. Higuchi, Y. Takeuchi, M. Miwa, Y. Yamamoto, K. Tsunemoto, G. Yoshizaki. 2011.
Colonization, proliferation and survival of intraperitoneally transplanted yellowtail seriola quinqueradiata spermatogonia in nibe croaker Nibea mitsukurii recipient.
Fisheries Science 77, 69-77.
=>Link to: PubMed Journal

G. Yoshizaki, K. Fujinuma, Y. Iwasaki, T. Okutsu, S. Shikina, R. Yazawa, Y. Takeuchi. 2011.
Spermatogonial transplantation in fish: A novel method for the preservation of genetic resources.
Comp. Biochem. Physiol. Part D Genomics Proteomics 6, 55-61.
=>Link to: PubMed Journal

森田哲朗・吉崎悟朗. 2011.
ニジマス初期胚を利用したヒトα1-アンチトリプシンの生産
水産育種 40(2), 113-120.
PubMed  Journal

Y. Yamamoto, N. Kabeya, Y. Takeuchi, K. Higuchi, T. Yatabe, K. Tsunemoto, R. Yazawa, T. Kawamura, G. Yoshizaki. 2011.
Establishment of a stable transgenic strain in a pelagic egg spawning marine teleost, Nibe croaker Nibea mitsukurii.
Aquaculture 313, 42-49.
PubMed Journal

Jangprai A, Boonanuntanasarn S, Yoshizaki G. 2011.
Characterization of melanocortin 4 receptor in Snakeskin Gourami and its expression in relation to daily feed intake and short-term fasting.
Gen. Comp. Endocrinol.173, 27-37.
=>Link to: PubMed Journal

矢澤良輔・竹内裕・岩田岳・壁谷尚樹・薦田章・吉崎悟朗. 2011.
70m3水槽を用いたクロマグロの陸上飼育.
水産増殖 59, 473-481.
PubMed  Journal


2010年

K. Nagasawa, S. Shikina, Y. Takeuchi, G. Yoshizaki. 2010.
Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout.
Biology of Reproduction 83, 597-606. PubMed Journal

S. Shikina, G. Yoshizaki. 2010.
Improved in-vitro culture conditions to enhance the survival, mitotic activity and transplatability of rainbow trout type A spermatogonia.
Biology of Reproduction 83, 268-276
=>Link to: PubMed Journal

G. Yoshizaki, M. Ichikawa, M. Hayashi, Y. Iwasaki, M. Miwa, S. Shikina, and T.Okutsu. 2010.
Sexual plasticity of ovarian germ cells in rainbow trout.
Development137, 1227-1230.
=>Link to: PubMed Journal

R. Yazawa, Y. Takeuchi, K. Higuchi, T. Yatabe, N. Kabeya, and G. Yoshizaki. 2010.
Chub mackerel gonads support colonization, and proliferation of intraperitoneally transplanted xenogenic germ cells.
Biology of Reproduction 82, 896-904.
=>Link to: PubMed  Journal

Y. Yamamoto, N. Kabeya, Y. Takeuchi, Alimuddin, Y. Haga, S. Satoh, T. Takeuchi, G. Yoshizaki. 2010.
Cloning and nutritional regulation of polyunsaturated fatty acid desaturase and elongase of the marine teleost nibe croaker, Nibea mitsukurii.
Fisheries Science 76, 463-472.
PubMed Journal

M. Kobayashi, Y. Hayakawa, W. Park, A. Banba, G. Yoshizaki, K. Kumamaru, H. Kagawa, H. Kaki, H. Nagaya, Y.C. Sohn. 2010.
Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larnae.
Gen. Comp. Endocrinol. 167, 379-386.
=>Link to: PubMed Journal

壁谷尚樹・奥津智之・矢澤良輔・樋口健太郎・長澤一衛・三森亮介・中田久・濱崎将臣・竹内裕・吉崎悟朗 2010.
サバ科魚類vasa cDNAのクローニングおよびその配列多型を利用したPCRによるクロマグロ生殖細胞検出系の構築
水産育種 39, 115-126.
PubMed  Journal


2009年

Y. Takeuchi, K. Higuchi , T. Yatabe , M. Miwa , and G. Yoshizaki.
Development of Spermatogonial Cell Transplantation in Nibe croaker, Nibea mitsukurii (Perciformes, Sciaenidae). 2009.
Biology of Reproduction 81, 1055-1063.
=>Link to: PubMed Journal

K. Nagasawa, Y. Takeuchi, M. Miwa, K. Higuchi, T. Morita, T. Mitsuboshi, K. Miyaki, K. Kadomura, G. Yoshizaki. 2009.
cDNA cloning and expression analysis of vasa-like gene in pacific bluefin tuna Thunnus orientails.
Fisheries Science757, 71-79.
=>Link to: PubMed Journal

A. Yano, K. von Schalburg, G. Cooper, BF. Koop, G. Yoshizaki. 2009.
Identification of a molecular marker for type A spermatogonia by microarray analysis using gonadal cells from pvasa-GFP transgenic rainbow trout (Oncorhynchus mykiss).
Mol. Reprod. Dev.76, 246-254.
=>Link to: PubMed Journal


2008年
Y. Yamamoto and G. Yoshizaki. 2008.
Heterologous gap junctions between granulosa cells and oocytes in Ayu(Plecoglossus altivelis): Formation and role during luteinizing hormone-dependent acquisition of oocyte maturational competence.
Journal of Reproduction and Development 54, 1-5.
=>Link to: PubMed  Journal

奥津智之・小林輝正・竹内裕・吉崎悟朗. 2008.
異種間生殖細胞移植実験におけるvasa遺伝子の種特異的プライマーを利用したドナー由来生殖細胞および精子の検出法
水産育種 37, 29-36.
=>Link to: PubMed  Journal

.S. Shikina, S. Ihara, G. Yoshizaki. 2008.
Culture conditions for maintaining survival and mitotic activity of rainbow trout transplantable A-type spermatogonia.
Molecular Reproduction and Development 75, 529-537.
=>Link to: PubMed Journal

Y. Hayakawa, T. Morita, W. Kitamura, S. Matsumoto, A. Banba, H. Nagaya, K. Komei Hotta, Y. C. Sohn, G. Yoshizaki, M. Kobayashi. 2008.
Biological activities of single-chain goldfish follicle-stimulating hormone and luteinizing hormone.
Aquaculture 274, 408-415.
=>Link to: PubMed Journal


2007年
Y. Yamamoto, G. Yoshizaki, T. Takeuchi, K. Soyano, R. Patino. 2007.
Role of Gap Junctions and Protein Kinase A during the Development of Oocyte Maturational Competence in Ayu (Plecoglossus altivelis)
General and Comparative Endocrinology 155, 789-795.
=>Link to: PubMed Journal

A. Yano, K. Suzuki, G. Yoshizaki. 2007.
Flow-Cytometric Isolation of Testicular Germ Cells from Rainbow Trout (Oncorhynchus mykiss) Carrying the Green Fluorescent Protein Gene Driven by Trout vasa Regulatory Regions.
Biology of Reproduction78, 151-158.
=>Link to: PubMed Journal

T. Okutsu, S. Shikina, M. Kanno, Y. Takeuchi, G. Yoshizaki. 2007.
Production of trout offspring from triploid salmon parents.
Science 317, 1517.
=>Link to: PubMed Journal

Y.Yamamoto, G.Yoshizaki,T.Takeuchi,K.Soyano,F.Itoh,and R.Patino. 2007.
Differential expression and localization of four connexins in the ovary of the ayu (Plecoglossus altivelis).
Mol. Reprod. Develop.
74
, 1113-1123.
=>Link to: PubMed Journal

Alimuddin, G.Yoshizaki, V.Kiron, .Satoh, and T.Takeuchi. 2007.
Expression of masu salmon Δ5-desaturase-like gene elevated of EPA and DHA biosynthesis in zebrafish.
Mar. Biotechnol.
 9, 92-100.
=>Link to: PubMed Journal

E.Sawatari, S. Shikina, T. Takeuchi, and G. Yoshizaki. 2007.
A novel transforming growth factor- β superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial preliferation in rainbow trout (Oncorhynchus mykiss).
Develop. Biol. 301, 266-275.
=>Link to: PubMed Journal


2006年

T. Kobayashi, Y. Takeuchi, T. Takeuchi, G. Yoshizaki. 2006.
Generation of viable fish from cryopreserved primordial germ cells.
Molecular Reproduction and Development 74, 207-213.
=>Link to: PubMed  Journal

T. Okutsu, A. Yano, K. Nagasawa, S. Shikina, T. Kobayashi, Y. Takeuchi, G. Yoshizaki. 2006.
Manipulation of fish germ cell: visualization, cryopreservation and transplantation.
Journal of Reproduction and Development 52, 685-693.
=>Link to: PubMed Journal

T. Okutsu, K. Suzuki, Y. Takeuchi, T. Takeuchi, G. Yoshizak. 2006.
Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional eggs in fish.
Proceedings of the National Academy of Sciences of the United States of America. 103, 2725-2729.
=>Link to: PubMed Journal


2005年
G. Yoshizaki, Y. Tago, Y. Takeuchi, E. Sawatari, T. Kobayashi, T. Takeuchi. 2005.
Green fluorescent protein labeling of primordial germ cells using a nontransgenic method and its application for germ cell transplantation in Salmonidae.
Biology of Reproduction 73, 88-93.
=>Link to: PubMed Journal

Alimuddin, G.Yoshizaki, V.Kiron, S.Satoh, and T.Takeuchi. 2005.
Enhancement of EPA and DHA biosynthesis by over-expression of masu salmon Δ6-desaturase-like gene in zebrafish.
Transgenic Res. 14, 159-165.
=>Link to: PubMed Journal

S.Boonanuntanasan, T.Takeuchi, and G.Yoshizaki. 2005.
High-efficiency gene knockdown using chimeric ribozymes in fish embryos.
Biochem. Biophys. Res. Commun.  336, 438-443.
=>Link to: PubMed Journal


2004年

S.Boonanuntanasan, G.Yoshizaki, K.Iwai, and T.Takeuchi. 2004.
Molecular cloning, gene expression in albino mutants and gene knockdown studies of tyrosinase mRNA in rainbow trout.
Pigment Cell Res. 17, 413-421.
=>Link to: PubMed Journal

T. Morita, G. Yoshizaki, M. Kobayashi, S. Watabe, and T. Takeuchi. 2004.
Fish egg as bioreactors:the production of bioactive luteinizing hormone in transgenic trout embryos.
Transgenic Res. 13, 551-557.
=>Link to: PubMed  Journal

Takeuchi, Y., Yoshizaki, G., Takeuchi, T. 2004.
Surrogate broodstock produces salmonids.
Nature 430, 629-630.
=>Link to: PubMed Journal

Kobayashi, T., Yoshizaki, G., Takeuchi, Y., and Takeuchi, T. 2004.
Isolation of highly pure and viable primordial germ cells from rainbow trout by GFP-dependent flow cytometry.
Molecular Reproduction and Development 67, 91-100.
=>Link to: PubMed  Journal


2003年
Kobayashi, T., Takeuchi, Y., Yoshizaki, G., and Takeuchi, T. 2003.
Cryopreservation of trout primordial germ cells: a novel technique for preservation of fish genetic resources.
Fish Physiology and Biochemistry 28, 479-480.
=>Link to: PubMed Journal

Morita, T., Yoshizaki, G., Kobayashi, M., and Takeuchi, T. 2003.
Production of biologically-acitve recombinant goldfish gonadotropins in transgenic rainbow trout.
Fish Physiology and Biochemistry 28, 473-474.
=>Link to: PubMed Journal

S.Boonanuntanasarn, G.Yoshizaki, and T.Takeuchi. 2003.
Specific gene silencing using samall interfering RNAs in fish embryos.
Biochem. Biophys. Res. Commun. 310, 1089-1095.
=>Link to: PubMed Journal

Takeuchi, Y., Yoshizaki, G., and Takeuchi, T. 2003.
Generation of live fry from intra-peritoneally transplanted primordial germ cells in rainbow trout.
Biology of Reproduction 69, 1142-1149.
=>Link to: PubMed Journal


2002年
Boonanuntanasarn, S., Yoshizaki, G., Takeuchi, Y., Morita, T., and Takeuchi, T. 2002.
Gene knock-down in rainbow trout embryos using antisense morpholino phosphorodiamidate oligonucleotides.
Marine Biotechnology 4, 256-266.
=>Link to: PubMed Journal

Takeuchi, Y., Yoshizaki, G., Kobayashi, T., and Takeuchi, T. 2002.
Mass isolation of primordial germ cells from transgenic rainbow trout carrying the GFP gene driven by the vasa gene promoter.
Biology of Reproduction 67, 1087-1092.
=>Link to: PubMed Journal

Yoshizaki, G., Takeuchi, Y., Tominaga, H., Kobayashi, T., and Takeuchi, T. 2002.
Visualization of primordial germ cells in transgenic rainbow trout carrying green fluorescent protein gene driven by vasa promoter.
Fisheries Science 68, 1067-1070.
=>Link to: PubMed Journal

Yoshizaki, G., Takeuchi, Y., Kobayashi, T., Ihara, S., and Takeuchi, T. 2002.
Primordial germ cells: the blueprint for a piscine life.
Fish Physiology and Biochemistry 26, 3-12.
=>Link to: PubMed Journal


2001年
Miwa, T., Yoshizaki, G., Naka, H., Nakatani, M., Sakai, K., Kobayashi, M., and Takeuchi, T. 2001. Ovarian steroid synthesis during oocyte maturation and ovulation in Japanese catfish.
Aquaculture 198, 179-191.
=>Link to:PubMed Journal

Takeuchi, Y., Yoshizaki, G., and Takeuchi, T. 2001.
Production of germ-line chimeras in rainbow trout by blastomere transplantation.
Molecular Reproduction and Development 59, 380-389.
=>Link to: PubMed  Journal

Yoshizaki, G., Shusa, M., Takeuchi, T., and Patino, R. 2001.
Gonadotropin-dependent oocyte maturational competence requires activation of the protein kinase A pathway and synthesis of RNA and protein in ovarian follicles of Nibe, Nibea mitsukurii (Teleostei, Sciaenidae).
Fish Physiology and Biochemistry 25, 201-208.
=>Link to: PubMed Journal

Yoshizaki, G., Patino, R., Thomas, P., Bolamba, D., and Chang, X. 2001.
Effects of maturation-inducing hormone on heterologous gap junctional coupling in ovarian follicles of Atlantic croaker.
General and Comparative Endocrinology 124, 359-366.
=>Link to: PubMed Journal


2000年
Gao, T., X. Zhang, G. Yoshizaki, and S. Watanabe. 2000.
Study on mitochondrial DNA sequences of Japanese mitten crab, Eriocheir japonica II. 12S rRNA.
Journal of Ocean University of Qindao 30, 43-47.
=>Link to: PubMed  Journal

Yoshizaki, G., Sakatani, S., Tominaga, H., Takeuchi, Y., and Takeuchi, T. 2000.
Isolation and characterization of rainbow trout vasa-like gene and its application to primordial germ cell’s marker.
Proc. 6th. Intl. Symp. Reprod. Physiol. Fish 260.
=>Link to: PubMed  Journal

Yoshizaki, G., Jin, W., Patino, R., Thomas, P., and Janecek, L. 2000.
Structural organization of the Atlantic croaker connexin 32.2 gene and its 5' flanking region.
Marine Biotechnology 2, 154-160.
=>Link to: PubMed  Journal

Yoshizaki, G., Takeuchi, Y., Sakatani, S., and Takeuchi, T. 2000.
Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoter.
International Journal of Developmental Biology 44, 323-326.
=>Link to: PubMed Journal

Yoshizaki, G., Sakatani, S., Tominaga, H., and Takeuchi, T. 2000.
Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineage.
Molecular Reproduction and Development 55, 364-371.
=>Link to: PubMed Journal

Gao, T., G. Yoshizaki, and S. Watanabe. 2000.
Study on partial sequences of mitochondrial DNA 12S rRNA gene of chinese mitten crab, Eriocheir sinensis.
Journal of Fisheries Science in China 7, 1-4.
=>Link to: PubMed  Journal

Takeuchi Y., Yoshizaki, G., and Takeuchi,T. 1999.
Green fluorescent protein as a cell labeling tool and a reporter of gene expression in transgenic rainbow trout.
Marine Biotechnology 1, 448-457.
=>Link to: PubMed Journal



著書

2010年度

吉崎悟朗, 2010.
「第6章 水産物の安定供給を目的とした技術開発」
シリーズ21世紀の農学 世界の食料・日本の食料. 養賢堂, pp.101-116.


2008年度


G. Yoshizaki, 2008.
Spermatogonial transplantation in fish: Production of trout offspring from salmon parents.
Fisheries for Global Welfare and Environment, 5th World Fisheries Congress 2008, pp. 209-219.

2007年度

森田哲朗・吉崎悟朗・小林牧人・渡部終五・竹内俊郎, 2007. 「ニジマス受精卵を用いたヒトのタンパク質生産技術の開発」バイオプロセスハンドブック バイオケミカルエンジニアリングの基礎から有用物質生産・環境調和技術まで. NTS,pp.485-489.

吉崎悟朗・竹内裕・奥津智之, 2007. 「第5章 単離生殖細胞からの魚類個体の作出:細胞を介した遺伝子導入技法の樹立をめざして」. シリーズ21世紀の農学 動物・微生物の遺伝子工学研究. 養賢堂,pp.77-95.

2006年度

吉崎悟朗, 2006. 「サケからマスをつくる:始原生殖細胞を用いた魚類の発生工学」. いざ生の扉へ:クローンとエピジェネティクスの新展開. アドスリー,pp.123-144.

2005年度 以前

吉崎悟朗, 2002. 魚類の遺伝子操作「動物発生工学」(岩倉洋一郎、佐藤英明、舘鄰、東條英昭 編),朝倉書店.

Y. Takeuchi, G. Yoshizaki, H. Tominag., T. Kobayashi, T. Takeuchi. 2001.
「Viasualization and isolation of live primordial germ cells aimed at cell mediated gene transfer in rainbow trout.」 In Aquatic Genomics (Eds. By Shimizu and Aoki), Springer

吉崎悟朗, 2000. トランスジェニック技法の水産育種への応用.(次世代の水産バイオテクノロジー.隆島編).成山堂書店,東京,pp.72-85.

吉崎悟朗・隆島史夫, 1997. 遺伝子導入魚の作出と外来遺伝子の発現「魚類のDNA」(青木・隆島・平野編)恒常星社厚生閣, 東京, pp80-98.

吉崎悟朗・R.Patino・隆島史夫, 1997. コネクシン(ギャップ結合タンパク質)遺伝子
「魚類のDNA」(青木・隆島・平野編)恒星社厚生閣, 東京, pp379-390.


 

東京海洋大学 吉崎研究室

〒108-8477
東京都港区港南4-5-7